Collection, gross characteristics and freezing of baboon semen.
نویسندگان
چکیده
This report deals with some gross characteristics of baboon (Papio sp.) semen ejaculated spontaneously or collected by electro-ejaculation. Details of the equipment used and the collection procedure are described and the results of freezing trials are presented. The animals were sedated with Sernylan (Parke, Davis and Co.) at a dose of 0\m=.\8mg/kg intramuscularly. They were then tied in an extended position while lying on their side on the collection table. The penis was cleaned with a mild detergent and then washed thoroughly with water. It was during this process that one of the males ejaculated on three occasions. These ejaculates are referred to as spontaneous ejaculates. The stimulator used was a trans-jector (Nicholson, Inc.) in which the voltage output was controlled by a regulator switch with Steps 1 to 7, with each step having a maximum voltage output of 2\m=.\4,3\m=.\2,4\m=.\5,7\m=.\5,9\m=.\5,10\m=.\5 and 13\m=.\5 volts, respectively, and a cycle frequency of 60/sec. The rectal probe used was designed and built in our laboratory and is 1\m=3/16\ in. in diameter and 10\m=1/4\ in. long, with twelve circular copper electrodes (\m=3/16\ in. wide) placed \m=3/16\ in. apart and the first ring (Ring 1) located 1 & in. from the tip of the probe. By means of an electrode
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ورودعنوان ژورنال:
- Journal of reproduction and fertility
دوره 20 2 شماره
صفحات -
تاریخ انتشار 1969